Fig. 4. Generation of marked brk1, brk2 and brk3 mutant sectors for mosaic analysis. (A) Relative locations of brk and marker loci are illustrated, along with the location of a chromosome-breaking Ds transposon used to generate marked brk2 sectors. (B) Cross-sectional view of a hypothetical white sector in a green leaf illustrating all types of sector boundaries analyzed. Horizontal lines denote upper and lower epidermis; black circles denote veins. Green represents wild-type tissue; white represents mutant tissue. (A) Lateral boundary in the epidermis overlying wild type mesophyll. (B) Transverse boundary with mutant epidermis directly overlying wild-type mesophyll. (C) Lateral boundary in the epidermis coinciding with a lateral boundary in the mesophyll. (D) Lateral boundary in the epidermis overlying mutant mesophyll. (E) Transverse boundary having at least one layer of mutant mesophyll separating mutant epidermis from an underlying wild-type cell layer.

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