Fig. 4. Ft, Ds and Atro control planar polarity in the eye imaginal disc. In all panels, clones are marked by the absence of GFP (green). Elav (blue) is a marker for all neuronal photoreceptor nuclei. All pictures are vertical projections of several confocal sections. (A,A') Loss of ft alters PP within and outside the clone. Bar (red) highlights the R1/R6 pair of photoreceptors. Black arrows indicates wild-type ommatidia, non-autonomously affected by the clone. (A') Arrows indicate ommatidia with D (blue) and V (red) PP. (B,B') Loss of ds alters PP within and outside the clone. ds^UA071 mutant clones and diagram of ommatidial polarity. Bar (red). Black arrows indicate wild-type ommatidia non-autonomously affected by the clone. (C,C') Loss of Atro alters PP within and outside the clone Atro³⁵ mutant clones and diagram of PP. The ß-Gal from m-lacZ, a marker for the R4 cell (red). The endogenous equator is indicated by a yellow line. Black arrows point to wild-type ommatidia, non-autonomously affected by the clone. In several clusters more than one cell expresses ß-Gal (yellow arrows). (D,E') fj transcription is controlled by Atro. Expression of fj-lacZ (red in D and E, white in D' and E') in wild-type (D,D') and in a Atro³⁵ mutant clones (E,E'). ß-Gal is strongly upregulated in Atro mutant tissue. Note that inside Atro clones fj-lacZ maintains its gradient-shape expression, with higher levels on the part of the clone closer to the midline.

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