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Fig. 2. Floral phenotypes in wild type, ufo-2 and different phases of phenotypic restoration in ufo-2 35S::UFOind. (A) Wild-type flower, with 4 sepals (not visible), 4 petals, 6 stamens (only five visible) and a pistil with 2 carpels. (B) ufo-2 flower with no petals and stamens The four outer sepals have been removed. Two filaments (arrows), 1 out of 2 staminoid sepal (arrow), 1 carpeloid structure (asterisk), and 2 out of 4 inner sepals are visible. The pistil contains two fused carpels. (C) Type I ufo-2 35S::UFOind flower with 4 first whorl sepals, 1 petaloid sepal (arrowhead), 1 staminoid petal (arrow), 1 petal, 8 stamens and a pistil with 3 unfused carpels. (D) Type I ufo-2 35S::UFOind flower with 5 first whorl sepals, 1 sepaloid petal (arrow), 1 petal, 1 staminoid petal (black arrowhead), 8 stamens and a pistil formed by 3 unfused carpels. (E) Lateral view of a type I ufo-2 35S::UFOind flower with the 4 first whorl sepals removed to reveal the inner 2 sepals (arrowheads). (F) Type II ufo-2 35S::UFOind flower with 4 sepals, 2 petals, 1 staminoid petal (arrow), 1 petaloid stamen (arrowhead), 5 stamens (only 2 clearly visible) and a central pistil with 3 partially unfused carpels. (G) Type III ufo-2 35S::UFOind flower with 4 sepals, 4 petals, 6 stamens and a pistil with two carpels. (H) Type IV ufo-2 35S::UFOind flower with 4 first whorl sepals, 1 petal, 6 stamens and a pistil with two carpels. (I) Lateral view of a type IV ufo-2 35S::UFOind flower with 2 of the first whorl sepals removed to reveal the 4 inner stamens and a pistil with 2 carpels. Note the absence of second whorl organs. (J) SEM of a type I ufo-2 35S::UFOind flower. One sepal has been removed to show the inner organs. (K) Detail of J showing 3 stamens in an outer whorl (arrows) and 3 stamens in an inner whorl (arrowheads). (L) Type I ufo-2 35S::UFOind flower with outer organs removed showing the central pistil unfused along two sides. One of the unfused margins has a thickening of the distal part with cell types similar to the anther (compare black and white arrowheads) and cells similar to the filament of the stamen (compare black and white arrows) form the proximal region. (M) Type I ufo-2 35S::UFOind flower with outer organs removed to expose a central pistil formed by three carpels completely unfused on one side. Arrowhead points to an abnormal ovule. (N) Detail of L showing an abnormally shaped ovule. (O) Type IV ufo-2 35S::UFOind flower. No visible petals are present between the sepal whorl and the stamens. (P) Dissected type IV ufo-2 35S::UFOind flower with the sepals removed. No reduced petal is visible. The arrow points to the insertion point of a removed sepal. The arrowheads indicate 2 stamens. A-I are at the same magnification. Bars: 1mm for A-M and O; 100 µm for N and P.





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