Fig. 5. Analysis of the N-terminal-proximal region of Dd-STATc. Identification of nuclear import signals in Dd-STATc. The approximate N-terminal half (residues 1-504) of Dd-STATc was fused upstream of GFP, to produce GFP:STATc1-504 and introduced into Dictyostelium cells. Stably expressing clones were selected and western transfer analysis confirmed that the fusion gene produced a fusion protein of the expected size. The cells were then analysed as in Fig. 4. (B) Analysis of the effect of the EXP region on the N-terminal-proximal import region. Construct GFP:STATc1-554 differs from construct GFP:STATc1-504 only in that it contains the EXP region. GFP:STATc1-554 was transformed into cells and they were analysed as described in Fig. 4. The panel at the right shows cells treated with LMB for 90 minutes, resulting in nuclear accumulation.

Return to article