Fig. 4. Disorganization of Sertoli cells in Dax1-deficient testis. In situ hybridization for Dhh (A,B), Amh (C,D) or Sox9 (E-H) reveals robust expression confined to the cords in wild-type testis, whereas expression is decreased and not clearly organized in Dax1-deficient testis. In situ hybridization for Dhh and Amh was performed at 12.5 dpc, and Sox9 was analyzed at 12.5 and 14.5 dpc. (I,J) Immunohistochemistry for Gata4 (green) and laminin (red) reveals decreased organization and differentiation of Sertoli cells at 13.5 dpc in Dax1-deficient testis (J) compared with wild-type males (I). Left panel, wild-type testis (A,C,E,G,I); right panel, Dax1-deficient testis (B,D,F,H,J).

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