Fig. 6. Curtailing Fms activity eliminates xanthophores and perturbs melanophore stripes thoughout development. (A-C) Examples of fms^174A individuals reared at 24°C to the sizes indicated (upper panels) then shifted to 33°C until an adult pigment pattern had formed (lower panels). (A) Larva shifted during early pigment pattern metamorphosis (7.6 mm SL) loses xanthophores and fails to develop normal adult stripes (14.3 mm SL, A') after 28 days at 33°C. (B) Larva shifted during middle stages of pigment pattern metamorphosis (8.9 mm SL) loses xanthophores and initial melanophore stripes degenerate (15.6 mm SL, B') after 28 days at 33°C. (C) Individual that has already attained a juvenile pigment pattern (13.5 mm SL) retains some xanthophores and a partial stripe pattern with more variably spaced melanophores (14.9 mm SL, C') after 14 days at 33°C. (Insets) Higher magnification views of boxed regions showing absence of xanthophores (A',B') or residual xanthophores (C' arrow). (D-I) Prolonged rearing at 33°C results in a complete loss of xanthophores Shown are sequential images of the same region on a representative fms^174A individual that had developed a juvenile pattern of melanophore stripes (18 mm SL) at 24°C (D), with times after shifting to 33°C of (E) 3 days, (F) 6 days, (G) 8 days, (H) 12 days and (I) 20 days. (Upper images) Low magnification showing melanophore distributions. (Lower images) Higher magnification showing depletion of xanthophores (arrow). (Inset) in G, high magnification showing melanophore debris indicated by arrow. Scale bars, (A,B) 1 mm, (C) 2 mm, (A'-C') 500 µm, (D-I) 250 µm.

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