Fig. 7. Chromatoblast and chromatophore death following Fms inactivation. (A) Two hours after shifting fms^174A mutants from 24°C to 33°C, an increase in TUNEL⁺ cells is observed in neural crest migratory pathways. Shown are superimposed fluorescence and bright-field images of TUNEL⁺ cells (arrow) adjacent to the dorsal neural tube during the stages of neural crest cell migration in a 28 h embryo. nt, neural tube. e, epidermis. m, myotome. B,C,D,E,F are brightfield images; B' and D' are corresponding fluorescence images of B and D. (B) In juvenile fish shifted from 24°C to 33°C, unpigmented TUNEL⁺ cells (arrow B') occur in the dermis where fms-expressing cells are found. s, scale. (C) Extensive chromatophore debris can be identified in the skin after shifting adult fish from 24°C to 33°C. Shown is a whole mount region of the caudal fin, with orange and black debris from xanthophores and melanophores, respectively. c, capillary. r, fin ray. (D) Extrusions in the superficial epidermis contain orange pigment. (D') Autofluorescence reveals presence of xanthophore-derived pteridine pigments. (E) Extrusion from the fin epidermis (arrow) contains debris of both xanthophores and melanophores. (F) Extrusion containing xanthophore-derived pigment on the fin of a wild-type adult. Scale bars: (A-C) 40 µm, (D,E) 20 µm.

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