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Fig. 5. BicC, Orb and Bru interact in vitro. (A) Immunoprecipitation experiments were performed on total ovarian extract using ${alpha}$ -Orb (lane 1), ${alpha}$ -BicC (lane 2) or ${alpha}$ -Hrp48 (lane 3) antibodies, or beads alone (lane 4). After precipitation, proteins were detected using ${alpha}$ -Orb antibodies. Equal amounts of extract were used in each sample. No Orb protein is retained on the beads alone (lane 4) or with ${alpha}$ -Hrp48 (lane 3) antibody. Orb is readily precipitated by ${alpha}$ -Orb (lane 1) and ${alpha}$ -BicC (lane 2) antibodies. (B) Bru protein is pulled down from total ovarian extract by the ${alpha}$ -Orb antibody. The same blot was probed with ${alpha}$ -Orb (1) and ${alpha}$ -Bru antibodies (2).

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