Fig. 3. Distribution of Maelstrom protein during oogenesis. (A) Left panel: three-dimensional projection of a partial confocal z-series stack showing a wild-type ovariole double-labeled (left) for Vasa (middle, green channel) and Maelstrom (right, red channel). Maelstrom colocalizes with Vasa in highly abundant perinuclear particles of all germline cells. Arrows indicate individual stem cell nuclei (SCN), nurse cell nuclei (NC) and an oocyte (OO). (B) From left to right: double labeling of Maelstrom (red) and nuclear Lamin (green) in a stage 4 egg chamber; enlargement of a single nurse cell nucleus; single Maelstrom and single Lamin channels. Maelstrom particles are closely apposed to the cytoplasmic face of the nuclear envelope. Scale bar: 10 µm. (C) An Aub-GFP germarium stained for Maelstrom and Vasa. Upper left: merged image of Vasa (green, upper right), Maelstrom (red, lower right) and Aub-GFP (blue, lower left) channels. Each discrete perinuclear particle labels for the three nuage components. Scale bar: 10 µm. (D) Localization of Vasa (green) and Maelstrom in a stage 10 egg chamber. Within nurse cells both proteins are present in nuage and cytoplasm. Maelstrom, unlike Vasa, shows no accumulation in the oocyte posterior. (E) Localization of Vasa and Maelstrom within a stage 2 oocyte nucleus. Panels from left to right: diagram of confocal field, with nurse cell nucleus (NCN) and oocyte nucleus (ON) indicated. Vasa (green in merged image) frequently localizes to intranuclear punctae (white arrow) ringed by concentrated Maelstrom (red in merged image). Scale bar: 2 µm. Anterior is towards the left in all panels. (F) Dorsal anterior corner of a stage 10B egg chamber labeled for 1B1 (green) and Maelstrom (red), which is present as diffuse staining in the oocyte nucleus. Scale bar: 20 µm.

Return to article