Fig. 4. Leptomycin B inhibits the nuclear export of Maelstrom. Ovaries were incubated for 20 minutes with or without 50 nM LMB, then stained for Vasa and Maelstrom. Two representative double-labeled ovarioles (A-D) and enlarged fields (E-H) are shown. The distribution of Vasa between nucleoplasm, nuage and cytoplasm of nurse cells is unperturbed during the 20 minute incubation without LMB (A, enlarged in E). There is a slight nuclear elevation of Vasa in LMB-treated samples (B, enlarged in F). Maelstrom maintains a normal distribution in the control ovariole (C, enlarged in G); upon LMB treatment (D, enlarged in H), Maelstrom is simultaneously reduced within cytoplasm of nurse cells and the oocyte, and enhanced within nuclei of the same cells. Anterior is towards the top in all panels. Scale bar: 20 µm in A-D.

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