Fig. 5. Dependencies of nuage particle assembly. Maelstrom (A1) and Vasa (A1') localization in wild-type, aub^HN2/N11 (A4, A4') and spn-E^616/hls125 (A5,A5') ovarioles. Maelstrom (A2) and Vasa (A2') localization in mael^M391/Df (3L)79E-F ovarioles; and Maelstrom localization in vasa ovarioles (A3). (Vasa signal in vasa ovarioles was not examined in this experiment, but in similar experiments performed, no signal is detected.) (B,C) Enlargements of nurse cells (B) and stem cells (C) from the correspondingly numbered panels in A. (A1) Distribution of Maelstrom in a wild-type ovariole. The highest concentration of Maelstrom is in perinuclear granules, evident in the germline from stem cells and cystoblasts (enlarged in C1) to nurse cells (enlarged in B1). In the maelstrom ovariole, virtually no Maelstrom immunoreactive signal is present (A2); yet Vasa is largely maintained in nuage (A2'). Maelstrom protein level in the germline of vasa null ovaries (A3, B3, C3) shows little preferential distribution to perinuclear particles in any cell type. (A4) Maelstrom in an aub^HN2/N11 ovariole. Maelstrom's preferential localization to perinuclear particles is abolished. Instead, Maelstrom often accumulates on membranes between germline cells [B4 (black arrow), C4]. (A5) Maelstrom in a spn-E^616/hls125 ovariole. Maelstrom shows no preferential accumulation in perinuclear particles in any cell type (B5,C5). Differential localization of Vasa to nuage is largely maintained in mael (A2', B2',C2'), aub^HN2/N11 (A4',B4',C4') and spn-E^616/hls125 (A5',B5',C5') backgrounds. Anterior is towards the top in all panels.

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