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Fig. 11. Local cellular movement correlates of (A) convergence and extension in the
wild-type embryo, and (B) of extension without convergence in the ntl
mutant. The sets of panels show time sequences, at 4 minute intervals, for the
notochord (WT)/axial (mutant) domains. For every cell in the field in turn
(`reference cell', the point at the center of each plot), we calculate the
displacements of all other notochord-domain cells relative to this reference
cell, i.e. the cell movement toward or away from the reference cell. This is
repeated for every cell in the notochord domain, each cell in turn taking the
role of a reference cell. The data are then averaged for each time point, and
the resulting averaged plots are shown. The arrows represent the average
directions and speeds (length of arrow) of these relative movements; their
placements on the grid are according to the positions of the cells relative to
the reference cell. Inward cellular movements (i.e. horizontal arrows point
towards the reference cell) correlate with convergence of the field, and
outward movements (vertical arrows point away from the cell) correlate with
extension. Inward movements are not sustained in the ntl mutant.
Positions along the x-axis reflect positions along the AP axis
relative to the reference cell while positions along the y-axis
reflect positions along the ML axis relative to the reference cell. See Movies
17 and 18 at
http://dev.biologists.org/supplemental/.