Fig. 5. MIB can account for all or most organized cellular movement within the wild-type notochord domain. Each purple line shows the observed movement pathway of individual cells over a 16 minute interval. The first 16 minutes of cell movement (A) and the last 16 minutes of cell movement (B, 4 hours later) of the recording used for Fig. 3 are shown. The yellow lines show the essentially random movements remaining after using the values of the convergent and extension rate constants to subtract the components of the cell movements due to MIB at every time point. At the beginning of the recording, before significant tissue shape changes have occurred (A), there is little difference between the location of the observed positions (purple) and the calculated positions with MIB removed (yellow). At the end of the 4 hour period, the observed field has undergone a significant shape change, convergence and extension (B, purple) but with MIB removed, the field has hardly changed shape at all. See Movie 6 at http://dev.biologists.org/supplemental/.

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