Fig. 6. Morphogenesis of the dorsal mesoderm is disrupted in the no tail/Brachyury mutant. Negative images of BODIPY-ceramide labeled preparations of wild type (A-C) and ntl mutants (E-G) at 9.5 (A,E), 10.2 (B,F) and 11 hpf (C,G). D1-5 show a through focus series (3 µm steps, shallow to deep) at the level of the second somite in another wild-type embryo at 11 hpf. D1 and D2 pass through the floor-plate region of the neural tube. D3 passes through the notochord-floor plate boundary. D4 and D5 pass through the notochord. In wild-type embryos, the notochord/somite boundary is apparent at 9.5 hpf (A), but in the ntl mutant a boundary between axial and paraxial domains begins to form only at 10.2 hpf (F). Cells become oriented along the ML axis, and exhibit a wedge-shape in the wild-type notochord-domain (B,C,D4,D5) but not in the mutant axial domain (E,F). Scale bar: 25 µm.

Return to article