Fig. 9. (A) Extensive cellular mixing along the ML axis occurs in ntl^-, but not in wild-type embryos. Fields are shown at 8, 8.8 and 9.5 hpf, with the spheres representing tracked cells color-coded to produce vertical stripes (30 µm wide) at the first time (8 hpf). To facilitate comparison with ntl^-, only the medial portion (size matched with the ntl^- field) of the tracked field is shown for the wild type. The stripes all narrow in the wild type and very little mixing is occurring between the stripes. In ntl^-, the stripes mix rather than narrow. (B) Cells move toward the midline in wild type, but move in a disorderly way with respect to the midline in ntl^-. Red and blue circles show cells moving to the right and left, respectively; green circles show cells with no left-right component to their movements at the representative time point illustrated (8.8 hpf). The center of each field corresponds the approximate midline for both A and B. See Movies 13-16 at http://dev.biologists.org/supplemental/.

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