Fig. 2. Genes from a known transcriptional cascade and from three previously characterized expression classes are all detected. (A) Published localization patterns for five transcription factors involved in specification of intestinal fate are depicted on a partial lineage diagram. skn-1, end-1 and end-3 expression patterns were determined using in situ hybridization; med-1/2 was determined using a combination of transgenic reporter and RT-PCR; and elt-2 was determined using antibody. The known regulatory interactions among these genes and proteins are shown along with the moving average time points of gene expression profiles. (B) Gene expression profiles for each of the five genes in A. med-1 and med-2 are treated as a single gene as there is only a single probe set on the chip to assay either and it does not distinguish between the two highly similar sequences. Colors correspond to those in A. (C) Gene expression profiles are shown for representatives of each of three previously characterized expression classes: vet-4, very early [embryonic] transcripts (vet); pos-1, Class I maternal; and tbb-2, Class II maternal.

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