Fig. 5. Defective spindles fail to associate with fusome in orbit⁶ mutants. (A-C) Germaria stained with anti-Orbit/Mast (red), anti--tubulin (green) antibodies and TOTO3 to reveal DNA (blue). (A) At metaphase, Orbit/Mast concentrates at spindle poles and localises along spindle MTs in wild-type stem cells. (B) Short bipolar (arrowheads) and monopolar (arrow) spindles seen at the tip of an orbit⁶ germarium. (C) Multiple short spindles at the tip of an orbit⁶ germarium. Arrowhead indicates a short bipolar spindle. (D-G) Germaria stained with anti-Orbit/Mast (red), anti--tubulin (green) and anti--spectrin (blue). (D) Wild-type stem cell at metaphase, showing the anterior spindle pole attached to the fusome. (E) Wild-type eight-cell germline cyst at metaphase with the spindles being attached to fusome through one of their poles. (F) Monopolar (arrow) and bipolar spindles that fail to associate with the fusome in an orbit⁶ germarium. (G) orbit⁶ germarium showing a monopolar spindle (arrow) close to the very apical region of the germarium, and a poorly organised germline cyst (arrowhead) situated more posteriorly. Both contain fusome pieces of different sizes. Scale bars: 5 µm A-E; 10 µm in F.

Return to article