Fig. 8. Localisation of CLIP-190 is disrupted in orbit⁶ mutants. (A-F) Germaria and egg chambers stained to reveal CLIP-190 (red), -Tubulin (green) and DNA (blue). The monochrome panels correspond to the staining pattern of CLIP-190. (A) Wild-type four-cell cyst at metaphase with CLIP-190 localising on the spindle and fusome. (B) Wild-type 16-cell stretching (arrowhead) and lens-shaped (arrow) cysts showing specific accumulation of D-CLIP-190. (C) Wild-type stage 6-7 egg chamber; CLIP-190 displays a punctate localisation along MT bundles (inset) and accumulates in the apical cortical region of the follicle cells. (D) Mitotic cyst in orbit⁶ germarium with no CLIP-190 on the spindles and a fusome-like body containing CLIP-190 (arrow) that contacts a spindle pole. (E) Egg chamber of a young orbit⁶ female with irregular microtubule bundles that show no accumulation of CLIP-190. (F) Egg chamber of an older orbit⁶ female with a few irregular microtubule bundles. Scale bars: 10 µm. (G) Western blots of total ovarian extracts immunoprecipitated with anti-CLIP-190 or anti-Orbit/Mast specific antibodies, subjected to western blotting and probed with the indicated antibodies.

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