Fig. 2. Distinct distribution of RNCAM isoforms on axons of OSNs and Z1-specific NADPHd-activity. Images were processed to show RNCAM immunoreactivity in red, RNCAM in situ hybridization signal in white, NADPHd-activity in yellow and nuclear counterstain in blue. (A-C) Anti-RNCAM immunohistochemistry analyses of nerve and glomeruli layers in a region of the OB innervated by Z1 axons lacking endogenous RNCAM expression in (A) control mice lacking RNCAM immunoreactivity, (B) GpiRNCAM transgenic mice with RNCAM immunoreactivity predominantly localized to the in nerve layer, and (C) TmRNCAM transgenic mice with RNCAM immunoreactivity localized to both nerve and glomerular layers. The broken line indicates the border between the two layers. (D) In situ hybridization analyses showing RNCAM expression in Z2-4; (E) NADPHd histochemistry analyzes showing a signal selectively confined to Z1 OSNs (Z1). Note the complementary signals on either side of the Z1/Z2 border (broken line). (F) NADPHd histochemistry analysis showing signal in the nerve and the glomerular layers of the OB. (G) Anti-RNCAM immunohistochemistry analysis showing RNCAM immunoreactivity in the nerve and the glomerular layers of the OB. Note the complementary signals on either side of the broken line. Experiments were carried out using serial coronal sections of OE (D,E) and OB (F,G).

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