Fig. 5. Mouse, human and chick interspecies comparisons reveal sequence conservation within the NCE. The nucleotide sequence of mouse fragment #17 (Fig. 3) is shown. Restriction sites used to generate internal deletion fragments #19 (BfaI and Bsu36I), #20 (SmaI and BfaI), #21 (SmaI and BanII) and #22 (BanII and BfaI) are underlined. , indicates the region deleted in construct #23. Sequences multimerised in fragment #24 are shown. The putative, head-to-head HMG box binding sites are indicated (HMG1/HMG2). Conserved sequences identified in the human genome and in the chick 1.7 kb NCE (Fig. 4) are aligned to the mouse sequence. Conserved residues are indicated as a dash in the human and chick sequences. Sequences satisfying the known Krox20 binding site matrix in all three species and footprinted by the protein in vitro (Fig. 6) are double underlined (K1 and K2). The G to C substitutions used to inactivate the Krox20 binding sites in constructs #18-23 are shown. Nucleotide numbering corresponds to the mouse sequence.

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