Fig. 4. Medial optic vesicle cells are viable but fail to constrict to form an optic nerve in vax1/vax2 morphants. (A) Schematic representation of the experiment (left) and dorsal view of an embryo with fluorescein uncaged in both left and right optic stalks (right). (B-G) Confocal images of living eyes, optic nerves and preoptic area of wild-type (B-D) and vax1-morpholino oligonucleotide (MO)/vax2-MO (E-G) embryos at the stages indicated bottom left. Arrowbars indicate the width of the optic stalk or nerve territory. Abbreviations: di, diencephalon; on, optic nerve; r, retina.

Return to article