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Fig. 5. PCNA and TUNEL labelling of colonic crypts. (A-D) PCNA staining of
Igf2 knockout (A), wild type (B), K:Igf2 samples (C) and.
negative control (D; wild-type sample with primary antibody omitted). The
proportion of the crypt that is PCNA positive is greater in the
K:Igf2. The knockout crypt shown is quite short but within the normal
range of sizes. Scale bars: 25 µm. Arrowheads indicate the upper limit of
PCNA-positive cells. (E,F) Crypt from a wild-type animal viewed for TUNEL (E;
showing a single apoptotic cell) and DAPI (F, showing all nuclei) staining.
Scale bars: 50 µm.
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