Fig. 2. Growth and lifespan of the mutants and wild type. (A) Growth curves. The worms were synchronized by allowing them to hatch for 4 hours and then grown at 20°C. Most worms became adults before 51 hours. Each point represents results on an independent culture plate. Average numbers of the examined animals at a point are as follows: N2, 32; ks60, 31; ks61, 25. Error bar indicates s.d. (B) Lifespan. The first time points represent the day when synchronized L1 larvae were put on NGM plates. Mean lifespan, standard deviation, number of examined animals excluding the missing ones/total number of examined animals, and number of tests performed independently are as follows: N2, 14.5±4.1 days, 207/300(6); ks60, 21.5±8.7 days, 80/150(2); ks61, 22.4±8.2 days, 143/200(4); daf-16(m26), 15.5±3.6 days, 131/150(3); daf-16(m26);ks61, 14.8±3.5 days, 88/150(3).

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