Fig. 1. Fgfr1 expression is efficiently abolished in the telencephalon of Foxg1-Cre;Fgfr1^flox/flox mutants. (A) Southern blot analysis showing that the floxed allele of Fgfr1 undergoes complete recombination in the telencephalon of Foxg1-Cre/+;Fgfr1fx mice. DNA was isolated from the dorsolateral telencephalon of E12.5 mice, digested with EcoRI, and probed with a 500 bp EcoRI-BamHI genomic DNA fragment containing exon 15. (B) RNA in situ hybridization analysis for Fgfr1 expression in a sagittal section through the telencephalon of an E12.5 control embryo. Fgfr1 is expressed throughout the neuroepithelium lining the telencephalic ventricle (tel) as well as in the facial mesenchyme (mes). Anterior is leftwards, dorsal is upwards. (B') Fgfr1 expression is lost from the telencephalon in mutant embryos. Scale bar: 0.25 mm. (C) Sagittal section through the nose of an E12.5 control embryo. Fgfr1 expression is widespread in the mesenchyme (mes) and, compared with the mesenchyme, is at a lower level in the olfactory epithelium (oe). (C') In the E12.5 mutant embryo, Fgfr1 expression is also lost from the olfactory epithelium, but not the surrounding mesenchyme, as expected given the recombination pattern observed previously with Foxg1-Cre mice (Hébert and McConnell, 2000). Scale bar: 0.1 mm.

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