Fig. 4. The specification of anterior cell types appears normal in the Fgfr1-deficient telencephalon. RNA in situ hybridization of sagittal sections of control (A,B) and mutant (A',B') brains with a probe against ephrin A5; anterior expression is maintained in mutants (arrow) at E12.5 (A,A') and E16.5 (B,B'; note that expression at this age is no longer organized in layers). Sections of E12.5 control (C) and mutant (C') brains probed for Pou3f1 shows expression in both control and mutant anterior telencephalon (arrow). (D) Gad67 marks interneurons migrating anteriorly into the olfactory bulb of P0 control (D) and mutant (D') brains. Sections of control (E,F) and mutant (E',F') brains at E12.5 (E,E') and E16.5 (F,F') hybridized to a Tbr1 probe; the accumulation of Tbr1⁺ neurons at the anterior tip of the brain occurs normally in mutants, although the cells are displaced ventrally (arrowheads). Scale bar in E', 0.4 mm; in F', 0.6 mm. (G,G') Nuclei of cells in the olfactory bulb region of E13.5 embryos stained with Syto11. (H,H') The same sections stained with antibodies against RC-2 to visualize radial glial cells. Scale bar: 0.05 mm.

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