Fig. 6. Expression of SEMA3C and neuropilin 2 in the developing chick embryo trunk. (A,C) Lateral views of wholemount in situ hybridisation for SEMA3C at stage 18 (A) and for neuropilin 2 at stage 20 (C). Somites anterior to the wing bud are shown (anterior is up, dorsal is right). SEMA3C expression in (A) is seen in the notochord (white arrows) and posterior halves of consecutive sclerotomes (white arrowheads). Neuropilin 2 expression in (C) is seen in the DRGs within the anterior half-sclerotomes (white arrowheads); black arrowheads indicate somite boundaries. Scale bars: 100 µm. (B,D) Transverse sections (30 µm) through the trunk of stage 19 embryos after whole-mount in situ hybridisation. (B) SEMA3C expression at the level of a posterior half-sclerotome. Expression is seen in the notochord, posterior half-sclerotome and ventral half of the neural tube. (D) Neuropilin 2 expression at the level of the anterior half-sclerotome. Expression is seen in the anterior half sclerotome in the region of DRG formation, and in the ventral neural tube. Scale bar: 100 µm. (E) Lateral view of whole-mount in situ hybridisation for neuropilin 2 at stage 24. Expression is seen in the anterior half-sclerotomes of recently formed somites (arrow) and in the DRGs forming more anteriorly (arrowheads). Scale bar: 1 mm. (F) Whole-mount in situ hybridisation for neuropilin 2 on a stage 26 embryo trunk. After in situ hybridisation, the trunk was hemisected and viewed from the neural tube side to help visualise expression. Weak neuropilin 2 expression is seen in the DRGs (arrows) and axons leaving the DRGs (arrowheads). Scale bar: 100 µm. as, anterior half-sclerotome; drg, dorsal root ganglion; nt, neural tube; n, notochord; ps, posterior half-sclerotome.

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