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Fig. 7. SEMA3C repels SCG but not early DRG axons in collagen gel co-cultures. (A,B) Stage 35 SCG explants cultured with COS cell aggregates transfected with (A) vector (VEC) alone (n=31) or (B) SEMA3C (SEMA3C) (n=24). (C,D) Stage 26-27 DRG explants cultured with COS cell aggregates transfected with (C) vector (VEC) alone (n=22) or (D) SEMA3C (SEMA3C) (n=18). (E) Histogram showing the mean repulsion score±s.e.m. Scores were assessed in cultures in which the explants lay between 150-450 µm of the COS cell aggregate. Asterisks indicate no significant repulsion. Scale bar: ~100 µm.





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