Fig. 2. The 5' deletion series to refine the minimal bam promoter. Schematic representations of the various deletion constructs are shown in A. Each construct's expression in germline stem cells (GSCs) and cystoblasts (Cb) is indicated in columns beside the appropriate construct schematic. The ability of the [Bam:GFP] constructs to rescue bam mutant flies was scored and is indicated in an additional column. Red and green rectangles represent the bam 5'-UTR and GFP, respectively. (B) Representative confocal images of the GFP expression pattern produced by individual constructs. Each panel contains a single optical section of a germarium in the plane of the terminal filament from animals expressing reporter from the indicated bam promoter fragment. The germarium in A was reacted with antibodies against Hts (red) and GFP (green) to show reporter expression in GSCs and cystoblasts. The arrowhead indicates the stem cell fusome located next to the contact point with cap cells, and the arrow points to the fusome located internally in a cystoblast. Asterisks indicate position of GFP-negative GSCs in A-E. (A) –898/+133-Bam:GFP; (B) –198/+133-Bam:GFP; (C) –96/+133-Bam:GFP; (D) –47/+133-GFP; (E) –168/+133-GFP; (F) –140/+133-GFP. gfp, green fluorescent protein.

Return to article