Fig. 6. Testing post-transcriptional effects of the bam silencer. (A) A schematic of the structure of a transgene in which a fragment of the bam region containing putative silencer sequences was joined to the nos promoter. The relative positions of oligonucleotides used for PCR primers are also shown above the schematic. (B) An example of the GFP expression pattern from the [nosP-bam5'-GFP-bam3'] is shown in a confocal optical section. The cell immediately adjacent to the base of the terminal filament, indicated by the arrow, is GFP-positive. (C) An agarose gel of products generated by RT-PCR of RNA isolated from [nosP-bam5'-GFP-bam3'] using oligonucleotides shown in A. The primer pairs used in the reactions are indicated on the right-hand side of the image.

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