Fig. 2. Mutations that affect cell number, size, and membrane protein distributions. All images are two-dimensional projections of confocal z-stacks showing mushroom body neuroblast clones homozygous for wild type (A), wkp¹ (B), 26D19 (C), 29B70 (D) and amz¹ (E). MARCM clones were induced in newly hatched larvae, fixed and stained for a membrane-targeted mCD8-GFP in wandering third instar larvae. Insets in A-D are high-magnification single confocal sections that show the details of the cell body staining. Image in B is overexposed to show the very weak labeling of axons and dendrites by the mCD8-GFP marker. The long arrow in E indicates an abnormally large cell. As the mushroom body is a three-dimensional structure, the dorsal lobe is sometimes almost perpendicular to the X-Y plane because of variation in mounting (see E). In this and all subsequent figures, samples are oriented such that dorsal is upwards and the midline is towards the right. Unless otherwise mentioned, arrowheads point to the calyx, which is the dendritic field of MB neurons. Brackets define the axonal peduncle before branching into the lobes. Horizontal and vertical arrows indicate the dorsal and medial lobes, respectively.

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