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Fig. 3. roadblock (robl) mutants affect axonal transport and neuroblast proliferation. (A,B) Neuroblast clones homozygous for roblMB examined in wandering third instar larva (A) and in adult (B). Red staining in B represents FasII immunoreactivity, which labels strongly the last-born class of {alpha}/ß neurons. The dorsal axons in roblMB neuroblast clones (green) are therefore {alpha}' axons, as they are not stained with FasII. (C) Wild-type mushroom body neuroblast clone double labeled with FasII (red). (D) A roblB neuroblast clone examined in wandering third instar larva. (E) A roblZ neuroblast clone examined in adult. (F,G) Axons of {gamma} neurons in adult medial lobe from single-cell clones of wild type (F) or roblZ (G). Long arrows in all images indicate accumulations of intense mCD8-GFP staining most probably because of accumulation of cargoes along the axons. Short oblique arrow in E indicates the cell body region of this neuroblast clone.





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