Fig. 4. short stop mutants cause multiple defects including neuronal polarity. (A,B) neuroblast clones homozygous for shot examined in larva (A) and adult (B). Long arrows point to overextended processes from the calyx. Short oblique arrow in A indicates axon termination in the peduncle as the intensity of mCD8-GFP staining progressively decreases as axons enter the lobe. Both images are overexposed to show the weak axonal lobes (composed of only the early-born neurons; the horizontal arrow indicates where the dorsal lobes should be) and the long arrow indicates the overextension from the calyx. Insets show the normal exposure of the cell body region. (C,D) Neuroblast clones with single staining of mCD8-GFP (C1,D1), Nod-ßgal (C2,D2) and double labeling (C3,D3) for shot³ (C) and wild type (D). In wild type, Nod-ßgal is confined to the cell bodies, calyx and sometimes the proximal part of the peduncle. In shot³ neuroblast clones, Nod-ßgal often intensely labels the entire peduncle and sometimes can be seen in the axonal lobes and distal end of the axons (double arrows in C, middle and right panels). Overextensions from the dendritic field (long arrow) are also strongly labeled with Nod-ßgal. (E) Quantification of the Nod-ßgal mislocalization phenotype. n=23 for wild type and n=14 for shot³.

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