Fig. 1. Mapping and cloning of the che-1 gene. (A) Genetic map position of che-1 on the linkage group I. (B) Physical map of the che-1 region. Indicated cosmids were tested for rescue of the defect in chemotaxis to NaCl of che-1 mutants. Rescue results are shown as + (positive) or - (negative), and the number of transformed lines that rescued the che-1 mutant phenotype as a fraction of the total number of independent lines tested are shown in parentheses. Each line was tested for chemotaxis at least with 20 individuals. When more than half of the animals show normal chemotaxis, the chemotaxis defect was regarded as being rescued. (C) Location of the che-1 gene. The top line and arrows show C55B7 near the left end and predicted transcripts based on the genomic sequence. PCR products and restriction fragments of the products were tested for rescue of the che-1(p696) mutant phenotype and the results are shown as in B. *The number of F1 transient transformants that rescued the che-1 mutant phenotype as a fraction of the total number of transient transformants tested.

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