Fig. 2. Dap is differentially regulated in oocytes and nurse cells. (A) A germarium stained with Dap antibody. Regions of the germarium are noted below. (B,C) Confocal sections of (B) an early stage 1 egg chamber and (C) a late stage 1 egg chamber stained for Dap (green) and Orb (red) to highlight the oocyte. Note that Dap is at high levels in all cyst nuclei in B, but has begun to cycle in C, as evidenced by the absence of Dap in some nuclei (asterisks). (D,E) Egg chambers stained with (D) Dap antibody and (E) DAPI to highlight nuclei. Consistent with the cycling of the Dap protein, nurse cells with high, medium and low levels of Dap are observed. Asterisks mark nuclei that are negative for Dap protein. (F) A stage-10B egg chamber stained for Dap (green) and Orb (red). Note that the nurse cells, which have exited the endocycle, contain uniformly high levels of Dap protein. (G) Double labeling for Dap (green) and BrdU (red) indicates that S phase takes place when Dap levels are low. (H) Corresponding DAPI staining. o, oocyte.

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