Fig. 4. GFP:PAR-2 and GFP:PAR-6 dynamics in nmy-2(RNAi) embryos. A,D,G,J are embryos during pronuclear migration; B,E,H,K are at pronuclear meeting; C,F,I are in late mitosis; L is a 2-cell embryo. GFP:PAR-2 remains off the cortex in the most severely affected embryos (A-C; Movie 15), unless par-6 is also removed (D-F, Movie 16). In more weakly affected embryos [nmy-2(RNAiClassII)], GFP:PAR-2 appears on the cortex around pronuclear meeting (H, Movie 18). Note PAR-2 accumulation in foci around pronuclei and/or spindles (C,H,I). GFP:PAR-6 remains uniformly distributed throughout the cortex even in the most weakly affected embryos (J-L, Movie 17).

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