Fig. 1. Localization of XOtx2 and XOtx5b in the developing Xenopus embryo. Whole-mount in situ hybridisation of embryos at stage 20 (A,D), stage 25 (B,E) and stage 31 (C,F) were probed with full-length cDNA of XOtx2 (A-C) and the 3' untranslated region of XOtx5b (D-F). (A) Before eye vesicle formation but after evagination, XOtx2 is expressed in both presumptive eye and anterior neural tube. (D) At this same stage, XOtx5b is found in the epiphysis (arrow) and cement gland but is distinctly absent from the eye field. (B) XOtx2 expression continues in the presumptive eye and forebrain (arrow). (E) Expression of XOtx5b just begins in a few cells of the eye and remains in the presumptive pineal (arrow) while cement gland expression recedes. (C) In a subsequent stage, XOtx2 expression becomes more pronounced in both the eye and the forebrain. (F) XOtx5b is found throughout the eye and in the singular pineal. Bars indicate 500 µm.

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