Fig. 4. In situ analysis of Dp1-deficient conceptuses at E8.5. Decidua containing a wild-type (A,D,G,J) and two Dp1-deficient (B-C,E-F,H-I,K-L) conceptuses were analyzed after serial sectioning. The genotypes were established for these embryos using laser-capture microdissection and then genomic PCR. (A-C) Hematoxylin and Eosin staining. (D-F) Immunohistochemical staining with TROMA1, which detects abundant trophectoderm-derived tissue in the wild-type conceptuses. (G-L) BrdU incorporation after a 30 minute in vivo labeling. (G-I) Low magnification; (J-L) high magnification. The embryo is the serpentine-like structure at the distal pole of each conceptus. The star denotes the position of the ectoplacental cone (EPC) and secondary trophoblastic giant (TG) cells at the proximal pole of the conceptus. The arrowheads denote the positions of trophoblast giant cells at the distal pole of the conceptus. Note the intensely BrdU-positive EPC and trophoblast giant cells surrounding the wild-type, but not the mutant embryos.

Return to article