Fig. 2. dock and Pak function autonomously in ONs for AL development. (A) ALs from animals with mosaic wild-type antennae: ey-FLP/+; Or47b-Gal4/+; FRT82/FRT82 M(3) arm-lacZ UNG. UNG stands for UAS-nysb::GFP. (B) ALs from animal with mosaic dock^P1 antennae: ey-FLP/+; dock^P1 FRT40/cycE FRT40; Or47a-Gal4 UNG/+. (C) ALs from animal with mosaic Pak¹⁶ antennae: ey-FLP/+; Or47b-Gal4/+; FRT82 Pak¹⁶ UNG/FRT82 M(3) arm-lacZ. (D-F) Animals expressing UAS-mCD8::GFP under the control of SG18.1-Gal4. (D) Confocal stereo pair of a 30 hAPF antenna double-stained with anti-mCD8 (green) and 22C10 mAb (red). Cells with a bipolar morphology, characteristic of ONs, express both GFP and Futsch. (E) Optical section of the adult ALs, showing GFP expression in the outer nerve layer (arrowhead) and in many glomeruli. Little GFP staining is observed outside of the AL. (F) Optical section at an equivalent plane as in E of an animal from which both the third segments of the antennae and the maxillary palps were surgically removed 6 days earlier. GFP staining in the outer nerve layer (arrowhead) and glomeruli is lost. (G-J) Expression of UAS-dock and UAS-Pak under the control of SG18.1-Gal4 rescues the mutant AL phenotypes. 3D reconstructions of ALs from dock (G) and Pak (I) mutants show that their ALs are mis-shapen and aglomerular. (H) Expression of the dock cDNA in dock^P1 homozygotes using SG18.1-Gal4 rescues the development of most of the glomeruli in the mutant. (J) Similarly, expression of Pak cDNA using SG18.1-Gal4 in Pak⁶/Pak¹¹ mutants strongly rescues the development of most of the glomeruli. (K) Quantification of the rescue of the dock and Pak AL phenotype by wild-type dock and Pak cDNAs. The frequencies with which four indicator glomeruli (VA1d, DA4, DM6 and VA6) were observed in the different genotypes are presented. Scale bars: in A, 25 µm for A-C,E-J; in D, 25 µm for D.

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