Fig. 5. Whole-mount RNA in situ hybridization staining of AME markers. (A,B) Lim1 is expressed in the ADE and primitive streak at E7.5, but is undetected in the AME of mutant embryos. (C,D) Hnf3b is expressed in the node and prechordal mesoderm in wild-type embryo at E7.5, but only extends a short distance anteriorly from the node in mutant embryos (arrow). (E,F) T is expressed in the primitive streak of the wild-type and mutant embryos. In the mutants, T is only expressed at a short distance from the node. (G,H). At E8.0, Hnf3b expression in mutants is normal in the node and most of the midline but is absent from the anterior head and foregut pocket region. (I-L) Analysis of the of prechordal plate markers Gsc and Dkk1 indicates that the E7.5 and E8.0 mutant embryos lack prechordal plate.

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