Fig. 3. Reduction in Rac activity inhibits tracheal cell rearrangement. Time-lapse observations of btl-Gal4, UAS-GFP-moesin/+ (A-E) and btl-Gal4, UAS-GFP-moesin/UAS-DRac1N17 (F-J) embryos. High-magnification views are shown for DB3 (D,E) and DB5 (I,J). White asterisks in F and G indicate absence of DB sprouts. Blue asterisks and broken red lines in D,E,I,J indicate DB cell nuclei and lumens, respectively. In an example shown in I and J, two cells at the tip of DB extend numerous filopodia and lead migration, but no additional cells follows, leaving only a thin stretch of the cytoplasm. Scale bars: in A, 40 µm for A-C; in F, 40 µm for F-H; in D, 20 µm for D,E; in I, 20 µm for I,J.

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