Fig. 4. The disrupted gene in Brd¹⁵/TM3 embryos encodes a Toll-like receptor. The wild-type tollo gene predicts a protein of 1346 amino acids. (Top) Conserved sequence domains identify the gene as a Toll-like Receptor (black rectangles, leucine-rich repeats, LRR; discontinuous black ovals, cysteine-rich domains, CR; gray oval, Toll homology signaling domain, TH; putative transmembrane domain, TM). (Bottom) Genomic sequence (nucleotides 17001 to 30000 from AE003531) contains a 4038 nucleotide ORF (gray box) within a single exon. Nucleotide 1 in the tollo ORF corresponds to nucleotide 18564 in AE003531. The boxed region (6735 nucleotides) was sequenced, either from genomic DNA derived from P1 phage (gray and white, nucleotides -17 to 5717) or by overlapping 3'-RACE (hatched, nucleotides 5398 to 6718). Arrow at the 5' end indicates a predicted promoter. Four consensus polyadenylation signals (A) are found within 5 kb of the first inframe stop codon. Sequence divergence in the TM3 chromosome is indicated by a broken line transposable element (TE). A polymorphic PstI genomic fragment confirms the site of divergence and is indicated in the aligned restriction map for the region (P, PstI; X, XbaI; H, HindIII). Probe 1 (EcoRI fragment) used for in situ hybridization and northern blots. Probe 2 (HindIII fragment) used for genomic Southern analysis.

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