Fig. 8. Transgenic expression of Tollo in Brd¹⁵ homozygotes rescues expression of the HRP epitope in the ventral nerve cord and in the peripheral nervous system. All panels show embryos probed with anti-HRP antibody. Whole-mount (A,D,G), ventral nerve cord (B,E,H) and three segments (C,F,I) of the peripheral nervous system are shown in OreR (A-C, stage 15), Brd¹⁵/Brd¹⁵ (D-F, stage 16) and tollo-Gal4; UAS-tollo; Brd¹⁵/Brd¹⁵ (G-I, stage 16) embryos. Transgenic Tollo rescues expression of the HRP epitope in the ventral nerve cord (G,H) but does not correct the head involution defect associated with the Brd¹⁵ deletion (compare arrowheads in D and G). HRP-epitope expression in elements of the peripheral nervous system first becomes apparent at stage 15 in OreR (short arrow in C). Loss of the HRP epitope in the peripheral nervous system of Brd¹⁵ homozygotes (F) is rescued by Tollo (short arrow in I). Tollo expression under control of hsp70-Gal4 without heat shock (J,K) induces HRP-epitope expression in the salivary gland (sg, paired structures at top and bottom of J,K) and rescues epitope expression in nearby sensory neurons (short arrows in K). The anterior end of dissected hsp70-Gal4; UAS-tollo; Brd¹⁵/TM3-lacZ embryos at stage 14 (J) and at stage 16 (K) are shown, anterior towards the left, after sequential lacZ activity staining (blue) and anti-HRP antibody staining. At dissection, the anterodorsal ectoderm of the head was sliced at the dorsal midline and flattened laterally. Subsequent removal of the embryonic brain lobes reveals the rescued sensory neurons (gg, garland gland; vnc, ventral nerve cord). Scale bar: 450 µm in A,D,G; 125 µm in B,E,H; 63 µm in C,F,I; 115 µm in J,K.

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