|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. Positional cloning of HST. The top line indicates the genetic map
of the region surrounding hst-1, as determined from an analysis of
F2 progeny from a cross to Ler. The number of recombinants
in each interval is indicated below the line. BACs that hybridized to the
lamba clone m262, which showed no recombination with hst-1,
are shown below this line. Hybridization of F9J1 to an EcoRV digest
of genomic DNA from hst-9 and Col revealed a missing fragment in
hst-9. When this EcoRV fragment was gel purified from F9L1
DNA and used to re-probe the blot, it hybridized to two fragments in
hst-9. These fragments are not visible in the blot hybridized with
the entire BAC because this blot was under-exposed in order to visualize as
many fragments as possible.
|
