Fig. 9. Two hybrid analysis of the interaction between HST and RAN1. Cells transformed simultaneously with the indicated vectors were selected for the presence of both constructs on medium lacking Leu and Trp, and then plated on a medium lacking Leu, Trp, His and Ade to test for the interaction between the AD and BD constructs. Cells containing the HST:BD and RAN1:AD vectors grew on this restrictive medium and expressed ß galactosidase (not shown). Cells containing RAN1:AD and either the hst-3:BD or N-HST:BD vectors grew very poorly or not at all on this medium, and did not express ß galactosidase.

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