Fig. 5. The longitudinal glial cells expressing repo and cut are misplaced in dri mutants. Dorsal view of dissected ventral nerve cord preparations of dri/CyO wgß gal (A,C,E) and dri¹/dri² homozygous (B,D,F) stage 16 embryos, immunochemically stained with anti-CT (A-B) or anti-REPO (C-F) antibodies and AP-conjugated (for REPO or CT in A-F) and HRP-conjugated (for ß-gal in CyO wgß-gal, A, C) secondary antibody. Anterior is towards the left. (A,C,E) dri heterozygotes exhibit the proper positions of two rows of glial cells either side of the midline. (B,D,F) Loss of DRI results in the misplacement of nuclei marked with CT (compare heterozygotes in A with dri homozygotes in B) or REPO (compare C with D). Higher magnification (E,F) more clearly shows that repo-positive nuclei of the longitudinal glial cells in dri homozygotes do not occupy a flat layer underlying the longitudinal connectives (F; arrowhead, nucleus in focus; arrows, mispositioned nuclei out of the focal plane) as in the wild type (E).

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