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Fig. 2. Expression of dally is regulated by Hh and En. (A-C) Misexpression
of the membrane-tethered form of Hh, HhCD2, induces ectopic expression of
dally. dally::lacZ expression was followed using
anti-ß-galactosidase antibody staining (A). Clones expressing
hhCD2 are marked by GFP in (B). (C) A merged image of A and B. Levels
of dally::lacZ are increased both in cells inside and
adjacent to the clones located in the A compartment (arrows), while clones in
the P compartment do not alter dally expression. (D-F) dally
is repressed by en in the P compartment. (D) Expression pattern of
dally::lacZ. (E) Positions of en-mutant clones are shown by
loss of GFP signal. (F) A merged image of D and E. dally expression
is significantly elevated in en-mutant clones induced in the P
compartment (arrow), indicating that dally is normally downregulated
by en. Arrowheads in A and D indicate the AP boundary.
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