Fig. 3. Expression of dally is repressed by Dpp signaling. (A-F) Clonal analysis for a severe tkv allele, tkv^a12, resulted in loss of the clones homozygous for tkv, because of growth defects, and preferential growth of their sister clones carrying two copies of the wild-type tkv in a genetic background heterozygous for tkv (one copy of wild-type tkv). The sister clones are marked by two copies of GFP (+/+), giving brighter signals than the single copy of GFP (+/–) (B,E). Wild-type (+/+) clones at the A/P border (A-C) and peripheral to the border (D-F) autonomously decrease the expression of dally::lacZ (arrows). (G-I) Clonal analysis for a mild tkv allele. dally::lacZ expression (G) is elevated in clones of cells homozygous for a partial loss-of-function tkv allele, tkv⁶ (arrows). (J-L) Effects of tkv^a12 mutation on dally::lacZ expression in the notum. Levels of dally expression (J) were significantly increased in tkv^a12 clones (arrows). Positions of clones are shown by loss of the GFP signal (H,K). (M-O) Effects of ectopically activated Dpp signaling on dally::lacZ expression. Expression of dally was repressed in the FLP-OUT clones that express a constitutively active form of tkv, tkv^Q253D (M). Positions of the FLP-OUT clones are marked by GFP (N). (C,F,I,L,O) Merged images of the two left-hand images on each row.

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