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Fig. 2. Zebrafish gli mutations block Hh signaling. (A) Wild-type
expression of the Hh target gene nk2.2 is unaffected by injection of
lacZ mRNA. (B,C) nk2.2 expression is regionally absent in
dtr and yot/gli2 mutant embryos (arrows). (D) Injection of
shh mRNA leads to an expansion of nk2.2 throughout the CNS
in wild-type embryos (arrowheads). (E,F) Over expression of shh does
not activate nk2.2 expression in defective regions of dtr
and yot/gli2 mutants (arrows), but nk2.2 expression is
expanded in unaffected regions (arrowheads). All panels show lateral views of
20-somite (19 hour) embryos, anterior to the left, eyes removed. di;
diencephalon; HB, hindbrain; MB, midbrain; MDB, mid-diencephalon boundary;
MHB, midbrain-hindbrain boundary; te, telencephalon.
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