Fig. 4. Multiple organelles associate with the Balbiani body. (A) An electron micrograph of the Balbiani body in the anterior region of a young oocyte. The residual fusome (white arrow), still rich in ER-like vesicles, lies at the anterior near the ring canals (white arrowheads) it formerly occupied. Numerous mitochondria are located adjacent to the fusome, while scattered Golgi stacks lie further below (asterisks). (B,C) Electron micrographs showing nearby sections of a single ring canal (white arrowhead) connecting a nurse cell (NC) and a young ooctye (O). Five centrioles or centriole pairs (numbered) lie within the material moving in to form the Balbiani body. (D) The anterior portion of an ovariole stained with anti--mannosidase 2 to reveal Golgi vesicles (green) and with 1B1 to show the fusome (red). The Golgi elements associate with the fusome beginning in region 2b (yellow, arrowhead), move into the oocyte with the Balbiani body (arrow), but in a slightly older oocyte (broken line) spread throughout the ooplasm. (E) COXI-reactive vesicles (green) associate with the fusome (red) in region 2b (yellow, arrowhead) and move into the oocyte as part of the Balbiani body (arrow). (F) COXI-positive particles (green) appear to be mitochondria because they are co-labeled with the mitochondrial ATP synthase marker (red). Broken circle, oocyte. (G) COXI-positive particles (green) are not co-labeled with a Golgi marker (red, -mannosidase 2). Broken circle, oocyte. Scale bars: in E, 10 µm for D,E; in A, 1 µm for A; in C, 1 µm for B,C; in G, 1 µm for F,G.

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