Fig. 5. Her5 activity does not control MH regional patterning. Whole-mount in situ hybridization at the 3-somite (E-H), 5-somite (A-C), and 24 hpf (D) stages for the expression of patterning and neurogenesis markers, as indicated (bottom left, color coded), following up- or down-regulation of Her5 activity. All panels are dorsal views of whole-mount (E-G) or flat-mounted (A-D,H) embryos, anterior to the top; arrows point to the MH junction. (A-D) MH patterning is not altered in transgenic hsp-her5 embryos (right in each panel) compared to non-transgenic siblings (left) by heat-shock during late gastrulation (A-C) or by repetitive heat-shocks (D). (E-G) MH patterning also remains unaltered in MO^her5-injected embryos (right in each panel) compared to controls (left). (H) Co-expression of ngn1 and pax2.1 (see also fluorescent view, inset) across the MHB in a single embryo upon MO^her5 injection demonstrates that neurogenesis and patterning can be uncoupled by Her5 activity.

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